Coding
Part:BBa_K3628009:Design
Designed by: Tianzuo Zeng Group: iGEM20_SMS_Shenzhen (2020-10-27)
HpaB
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 67
Illegal PstI site found at 1408 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 67
Illegal PstI site found at 1408 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 83
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 67
Illegal PstI site found at 1408 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 67
Illegal PstI site found at 1408 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We use this after T7 promoter
Source
BL21 Genome